Caspase-3(P10)

Catalog Number: ASA-B0294

Lead time: 1 week

$299.00

Qty:

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
caspase 3, apoptosis-related cysteine peptidase	Polyclonal	IgG	Rabbit	Human	IHC-P, ICC, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-3(P10)(220-236aa CAMLKQYADKLEFMHIL).

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	CASP3
Protein	Caspase-3(CASP-3)
Uniprot ID	P42574
Function	Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
Tissue Specificity	Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Sub-cellular localization	Cytoplasm.
Sequence Similarities	Belongs to the peptidase C14A family.
Aliases	A830040C14Rik antibody\|Apopain antibody\|Apopain precursor antibody\|CASP 3 antibody\|CASP-3 antibody\|CASP3 antibody\|Casp3a antibody\|Caspase 3 antibody\|Caspase 3 apoptosis related cysteine protease antibody\|Caspase 3 p12 subunit antibody\|Caspase 3, apoptosis-related cysteine peptidase antibody\|Caspase 3, apoptosis-related cysteine protease antibody\|Caspase 3, apoptosis-related cysteine protease a antibody\|Caspase-3 subunit p12 antibody\|Caspase3 antibody\|CC3 antibody\|CPP 32 antibody\|CPP-32 antibody\|CPP32 antibody\|CPP32B antibody\|Cysteine protease CPP32 antibody\|EC 3.4.22.56 antibody\|Human cysteine protease CPP32 isoform alpha mRNA complete cds antibody\|ICE3 antibody\|LICE antibody\|mldy antibody\|OTTHUMP00000165052 antibody\|OTTHUMP00000165053 antibody\|OTTHUMP00000165054 antibody\|PARP cleavage protease antibody\|Procaspase3 antibody\|Protein Yama antibody\|SCA 1 antibody\|SCA-1 antibody\|SCA1 antibody\|SREBP cleavage activity 1 antibody\|Yama antibody\|Yama protein antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human	AssaySolution's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human	AssaySolution's IHC/ICC Detection kit
Immunocytochemistry	0.5-1μg/ml	Human	AssaySolution's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information

Anti-Caspase-3(P10), ASA-B0294, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

Anti-Caspase-3(P10), ASA-B0294, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SMMC Cell Lysate

Anti-Caspase-3(P10), ASA-B0294, IHC(P)
IHC(P): Human Lung Cancer Tissue

Caspase-3(P10)

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification

Immunogen

Properties

Form

Size

Contents

Concentration

Storage

Additional Information Regarding the Antigen

Gene

Protein

Uniprot ID

Function

Tissue Specificity

Sub-cellular localization

Sequence Similarities

Aliases

Application Details