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Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
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Gene ID
|
16819 |
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Gene Symbol
|
NGAL |
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Species
|
Mouse |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of Mouse NGAL . No significant cross-reactivity or interference between Mouse NGAL and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
|
15.6 -- 1000 pg/ml |
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Sensitivity
|
12.5 pg/ml |
|
Inter Assay
|
CV%<10% |
|
Intra Assay
|
CV%<8% |
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Assay Type
|
Sandwich ELISA |
|
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
|
50-100ul |
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Applications
|
ELISA |
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Typical Data
|
ELISA: Mouse NGAL ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
|
Mouse neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, 24p3, SIP24, uterocalin
and siderocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic
molecules. 24p3 was observed to be dramatically upregulated in kidney cell cultures infected with SV40
and subsequently identified as a lipocalin. Unlike human NGAL, it is an acute phase protein upregulated
in the liver. but like its mammalian homologues it is upregulated and secreted by the kidney in response
to ischemic or nephrotoxic injury. It is also upregulated in the involuting uterus and mammary gland. |
