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Storage
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Store the unopened product at 2 - 8° C. Protect from light. Do not use past
expiration date.
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Gene ID
|
25296 |
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Gene Symbol
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BMP-4 |
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Synonym
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BMP-2B; BMP2B1; BMP2BMCOPS6; BMP4; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; OFC11; ZYME |
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Species
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Rat |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of rat BMP-4. No significant cross-reactivity or interference between rat BMP-4 and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
|
0.624 ng/ml - 40 ng/ml |
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Sensitivity
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0.5 ng/ml |
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Inter Assay
|
CV%<10% |
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Intra Assay
|
CV%<8% |
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Assay Type
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Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
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50-100ul |
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Applications
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ELISA |
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Typical Data
|
ELISA: Rat BACE-1 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
|
Beta-secretase 1 (BACE1), also known as beta-site amyloid precursor protein cleaving enzyme 1, beta-site APP cleaving enzyme 1, membrane-associated aspartic protease 2, memapsin-2, aspartyl protease 2, and ASP2, is an enzyme that in humans is encoded by the BACE1 gene. BACE1 is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells. The transmembrane protein contains two active site aspartate residues in its extracellular protein domain and may function as a dimer. |
