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Storage
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Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
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Gene ID
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116590 |
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Gene Symbol
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ERK2 |
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Synonym
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EC 2.7.11; EC 2.7.11.24; ERK; ERK2; ERK-2; ERK2MAP kinase isoform p42; ERT1; Extracellular signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAPK 1; MAPK1; MAPK2; mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; p38; p40; p41; p41mapk; p42mapk; p42-MAPK; PRKM1; PRKM1MAPK 2; PRKM2; protein tyrosine kinase ERK2 |
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Species
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Rat |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of Rat Total ERK2. No significant cross-reactivity or interference between Rat Total ERK2and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
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78.125--5000 pg/ml |
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Sensitivity
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62.5 pg/ml |
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Inter Assay
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CV%<10% |
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Intra Assay
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CV%<8% |
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Assay Type
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Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
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50-100ul |
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Applications
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ELISA |
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Typical Data
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ELISA: Rat Total ERK2ELISA Kit (Colorimetric) These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
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ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44 and 42 kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. |
