YIPF3 Antibody

Catalog Number: ASA-B2005
Lead time: 1 week
$299.00

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

Yip1 domain family, member 3 Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human YIPF3(47-61aa FEDMGELHQRLREEE), identical to the related rat and mouse sequences.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

YIPF3

Protein

Protein YIPF3

Uniprot ID

Q9GZM5

Function

Involved in the maintenance of the Golgi structure. May play a role in hematopoiesis.

Tissue Specificity

Expressed by nucleated hematopoietic cells (at protein level).

Sub-cellular localization

Cell membrane; Multi-pass membrane protein. Cytoplasm. Golgi apparatus, cis-Golgi network membrane; Multi-pass membrane protein. Note: Localization to the cytoplasm or to the cell membrane is developmentally and ontogenetically regulated.

Sequence Similarities

Belongs to the YIP1 family.

Aliases

Killer lineage protein 1 antibody|Natural killer cell-specific antigen KLIP1 antibody|Protein YIPF3 antibody|YIP1 family member 3 antibody|Yipf3 antibody|YIPF3_HUMAN antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat MouseAssaySolution's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Rat MouseAssaySolution's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-YIPF3 antibody, ASA-B2005, Western blotting
All lanes: Anti YIPF3 (ASA-B2005) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Thymus Tissue Lysate at 50ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: SMMC Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 48KD
Observed bind size: 35KD
Anti-YIPF3 antibody, ASA-B2005, IHC(P)
IHC(P): Rat Testis Tissue